What's this?
A collection of webpages describing SILAC (Stable
Isotope Labeling
with Amino acids in Cell
culture), a general method for mass spectrometric-based quantitative
proteomics that incorporates the quantitative label through metabolic
incorporation. This web resource serves to supplement
publications from CEBI that utilize SILAC and to provide additional
information for those wishing to use this approach for their own
projects.
Stable isotope labeling (SIL) has been used
extensively for quantitative applications in the small molecule field
in the pharmaceutical industry for many years.
SILAC builds upon work previously described
where stable isotope enriched media was used for the cultivation of
bacteria or yeast (Lahm HW and Langen H Electrophoresis 2000, Oda Y and
Chait BT PNAS 1999). Several independent groups have
described the use of stable isotope amino acid labeling for increase
confidence in peptide identification in database searching.
Another acronym coined by Xian Chen
and colleagues from Los Alamos for this stable isotope labeling method
is AACM
for 'Amino Acid Coded Mass Tagging'. We first describe SILAC
for mammalian cell culture and apply it to a study of protein
expression changes during muscle differentiation in 2002.
The pages are composed of:
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